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課程名稱 |
冷凍電子顯微鏡導論
Introduction to Cryogenic Electron Microscopy |
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開課學期 |
109-1 |
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授課對象 |
理學院 化學所化學生物學組 |
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授課教師 |
陳振中 |
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課號 |
Chem5117 |
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課程識別碼 |
223 U1610 |
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班次 |
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學分 |
3.0 |
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全/半年 |
半年 |
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必/選修 |
選修 |
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上課時間 |
星期二2(9:10~10:00)星期四3,4(10:20~12:10) |
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上課地點 |
化121化121 |
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備註 |
本課程中文授課,使用英文教科書。
限學士班三年級以上 且 限本系所學生(含輔系、雙修生)
總人數上限：30人 |
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Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/1091Chem5117_cryoEM |
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課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
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課程大綱
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課程概述 |
This graduate course aims to give an introduction to the technique of cryo-EM, with emphasis on the underlying physical principles and the use of RELION for 3D reconstruction of protein structure. |
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課程目標 |
The following topics will be covered:
1. Diffraction principles;
2. Image formation in transmission electron microscopy;
3. Procedure in cryo-EM experiments;
4. Principles of 3D reconstruction;
5. RELION calculations. |
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課程要求 |
For practical calculations of RELION, priority will be given to those students who need cryo-EM for their research projects.
Some basic knowledge of calculus and linear algebra are required; hands-on experience in TEM is helpful. |
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預期每週課後學習時數 |
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Office Hours |
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指定閱讀 |
待補 |
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參考書目 |
Grant Jensen (ed.), Methods in Enzymology, 2010, volume 481-483.
Michael Moody, Structural Biology using Electrons and X-rays, 2011.
David Williams and Barry Carter, Transmission Electron Microscopy, 2009. |
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評量方式
(僅供參考) |
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No. |
項目 |
百分比 |
說明 |
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1. |
RELION calculations |
100% |
For those who have access to RELION calculations |
2. |
Presentation and report |
100% |
For those who do not have access to RELION calculations |
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